soluble fusion protein gpc3 (TaKaRa)
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soluble fusion protein gpc3
Soluble Fusion Protein Gpc3, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+fusion+protein+gpc3/Protein+Molecular+Weight+Marker/pm30896845-57-15-27
Average 94 stars, based on 512 article reviews
Soluble Fusion Protein Gpc3, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+fusion+protein+gpc3/Protein+Molecular+Weight+Marker/pm30896845-57-15-27
Average 94 stars, based on 512 article reviews
soluble fusion protein gpc3 - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3."
Article Title: Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3.
Journal: Molecular medicine reports
doi: 10.3892/mmr.2019.10019
Figure Legend Snippet: Figure 3. Expression, purification and identification of recombinant GPC3 protein. (A) Induction expression of recombinant GPC3 protein by SDS‑PAGE analysis. Lane M, protein molecular weight marker; Lane 1, non‑induction of GPC3; lane 2, induction of GPC3. (B) SDS‑PAGE analysis of purification of recombinant GPC3 protein. M, standard protein molecular weight; lane 1, purified protein. (C) Confirmation of the recombinant protein by western blot analysis. The primary antibody was rabbit anti‑His‑tag monoclonal antibody. M, standard protein molecular weight; lane 1, purified protein. GPC3, glypican‑3.
Techniques Used: Expressing, Purification, Recombinant, Molecular Weight, Marker, Western Blot
Related Articles
Recombinant:Article Title: Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3. Article Snippet: PCR fragments were digested using BamHI and XhoI restriction enzymes (10 U/μl; Takara Bio, Inc.) for 1 h at 37 ̊C and then cloned into the BamHI- and XhoI-digested pET16b vector ecoding a His‐tag (Novagen; Merck KGaA); successful cloning was confirmed by sequencing (Sangon Biotech Co., Ltd.). .. The recombinant plasmid was transfected into E. coli BL21 (DE3, Novagen; Merck KGaA) with the Plasmid Preparation:Article Title: Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3. Article Snippet: PCR fragments were digested using BamHI and XhoI restriction enzymes (10 U/μl; Takara Bio, Inc.) for 1 h at 37 ̊C and then cloned into the BamHI- and XhoI-digested pET16b vector ecoding a His‐tag (Novagen; Merck KGaA); successful cloning was confirmed by sequencing (Sangon Biotech Co., Ltd.). .. The recombinant plasmid was transfected into E. coli BL21 (DE3, Novagen; Merck KGaA) with the Transfection:Article Title: Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3. Article Snippet: PCR fragments were digested using BamHI and XhoI restriction enzymes (10 U/μl; Takara Bio, Inc.) for 1 h at 37 ̊C and then cloned into the BamHI- and XhoI-digested pET16b vector ecoding a His‐tag (Novagen; Merck KGaA); successful cloning was confirmed by sequencing (Sangon Biotech Co., Ltd.). .. The recombinant plasmid was transfected into E. coli BL21 (DE3, Novagen; Merck KGaA) with the Control:Article Title: Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3. Article Snippet: PCR fragments were digested using BamHI and XhoI restriction enzymes (10 U/μl; Takara Bio, Inc.) for 1 h at 37 ̊C and then cloned into the BamHI- and XhoI-digested pET16b vector ecoding a His‐tag (Novagen; Merck KGaA); successful cloning was confirmed by sequencing (Sangon Biotech Co., Ltd.). .. The recombinant plasmid was transfected into E. coli BL21 (DE3, Novagen; Merck KGaA) with the |